Custom Fluidic Solution for PCR
Precision Droplets, Powerful Results - Find out how IMI enabled high-efficiency DNA sample preparation for digital Polymerase Chain Reaction (PCR)
Challenges with complex flow paths, inconsistent droplet in PCR?
A life science customer set out to develop a DNA sample handling and preparation system capable of generating precise droplets of PCR oil-based reagent loaded with DNA. These droplets needed to be dispensed into well plates and seamlessly transferred to a digital PCR system for replication.
The challenge lay in designing a compact, integrated unit that could interface directly with disposable reagent bottles, manage complex fluid paths, and deliver consistent droplet formation—all while maintaining high throughput and reliability.
IMI’s Solution: Integrated Fluidics Manifold for Seamless Dispensing
IMI partnered with the customer to co-develop a customized fluidic assembly built around a 5-layer acrylic manifold. This innovative solution featured:
Chipsol On/Off valves, Media Separated valves, and a FLATPROP actuator for precise control.
Integrated sensors, fittings, and PCBs for real-time monitoring and automation.
Dual pressure chambers enabling an air-over-liquid system to extract reagent and direct it to a dedicated dispense head.
Direct bottle-to-manifold interface for simplified reagent loading.
This assembly allowed the dispense head to auto-fill well plates with high accuracy, reducing manual intervention and ensuring consistent droplet formation.
Key Benefits:
Reduced instrument production time through modular design.
Lower inventory complexity with integrated components.
Fewer field service claims due to robust engineering.
Improved operational efficiency for end users.
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